|本期目錄/Table of Contents|

[1]李磊,韓成,王宵宵,等.镉脅迫下轉基因水稻對根際土壤微生物的影響[J].江蘇農業科學,2019,47(14):282-287 .
 Li Lei,et al.Effect of transgenic rice on rhizospheric soil microorganisms under cadmium stress[J].,2019,47(14):282-287 .
點擊複制

镉脅迫下轉基因水稻對根際土壤微生物的影響(PDF)
分享到:

《江蘇農業科學》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期數:
2019年第14期
頁碼:
282-287
欄目:
資源與環境
出版日期:
2019-08-10

文章信息/Info

Title:
Effect of transgenic rice on rhizospheric soil microorganisms under cadmium stress
作者:
李磊12 韓成1 王宵宵12 洪鑫12 劉標3 鍾文輝1
1.南京師範大學地理科學學院/江蘇省物質循環與汙染控制重點實驗室,江蘇南京 210023; 2.南京師範大學環境學院,江蘇南京 210023;
3.生態環境部南京環境科學研究所/國家環境保護生物安全重點實驗室,江蘇南京 210042
Author(s):
Li Leiet al
關鍵詞:
轉基因水稻細菌群落根際土壤镉脅迫
Keywords:
-
分類號:
S154.3;Q938.1
DOI:
-
文獻標志碼:
A
摘要:
爲了探究镉脅迫下轉基因水稻對根際土壤微生物的影響,以抗蟲轉基因水稻華恢1號(HH1)及其親本非轉基因水稻明恢63(MH63)爲研究材料,設置不同Cd2+濃度(0、3.5、60.0、240.0 mg/kg)脅迫進行盆栽試驗。結果表明:與Cd0相比,Cd3.5處理對水稻農藝性狀無顯著影響;Cd240.0處理顯著降低了株高、産量;與Cd0處理相比,Cd60.0和Cd240.0處理下HH1水稻根際土壤細菌群落組成中Proteobacteria(變形菌門)、Acidobacteria(酸杆菌門)、Actinobacteria(放線菌門)的相對豐度顯著降低;MH63水稻中Acidobacteria的相對豐度顯著升高而Bacteroidetes(擬杆菌門)的相對豐度顯著降低。Cd脅迫顯著降低了水稻根際土壤細菌群落豐度及多樣性。環境因子分析顯示,Cd脅迫後水稻根際土壤pH值、總氮含量、铵態氮含量的變化是影響根際土壤細菌群落結構的主要原因。
Abstract:
-

參考文獻/References:

[1]Borah P,Singh P,Rangan L,et al. Mobility,bioavailability and ecological risk assessment of cadmium and chromium in soils contaminated by paper mill wastes[J]. Groundwater for Sustainable Development,2018,6:189-199.
[2]吳玉峰. 廣西典型高背景镉地區的生態風險評價[D]. 南甯:廣西師範學院,2016.
[3]Applications A B. Global status of commercialized biotech/GM crops[EB/OL]. [2018-01-10]. http://www.isaaa.org/kc,2004.
[4]劉志誠,葉恭銀,胡萃. 抗蟲轉基因水稻和化學殺蟲劑對稻田節肢動物群落的影響[J]. 應用生態學報,2004,15(12):2309-2314.
[5]Hu T Z,Zhu S S,Tan L L,et al. Overexpression of OsLEA4 enhances drought,high salt and heavy metal stress tolerance in transgenic rice (Oryza sativa L.)[J]. Environmental and Experimental Botany,2016,123:68-77.
[6]Zhao F Y,Wen L,Zhang S Y. Different responses of plant growth and antioxidant system to the combination of cadmium and heat stress in transgenic and non-transgenic rice[J]. Journal of Integrative Plant Biology,2009,51(10):942-950.
[7]Li C H,Zhu J G,Zeng Q,et al. Different responses of transgenic Bt rice and conventional rice to elevated ozone concentration[J]. Environmental Science and Pollution Research,2017,24(9):8352-8362.
[8]Liang Y,Liu F,Li J,et al. Coexistence of Bacillus thuringiensis (Bt)-transgenic and conventional rice affects insect abundance and plant fitness in fields[J]. Pest Management Science,2018,74(7):1646-1653.
[9]陳欣瑤,楊惠子,陳楸健,等. 重金屬脅迫下不同區域土壤的生態功能穩定性與其微生物群落結構的相關性[J]. 環境化學,2017,36(2):356-364.
[10]Liu B,Zeng Q,Yan F M,et al. Effects of transgenic plants on soil microorganisms[J]. Plant and Soil,2005,271(1/2):1-13.
[11]Liu W,Lu H H,Wu W X,et al. Transgenic Bt rice does not affect enzyme activities and microbial composition in the rhizosphere during crop development[J]. Soil Biology and Biochemistry,2008,40(2):475-486.
[12]陳敏,應文荷. 轉Bt水稻與常規水稻根際土壤細菌類群的比較研究[J]. 杭州師範學院學報(自然科學版),2005,4(4):290-292.
[13]Wang Z J,Deng H,Chen L H,et al. In situ measurements of dissolved oxygen,pH and redox potential of biocathode microenvironments using microelectrodes[J]. Bioresource Technology,2013,132:387-390.
[14]陳希. 茶園根際土壤與植物體養分對酸沉降的響應[D]. 南昌:南昌大學,2015.
[15]鮑士旦. 土壤農化分析[M]. 3版.北京:中國農業出版社,2000:232-248.
[16]薛銀剛,江曉棟,孫萌,等. 基于高通量測序的冬季太湖竺山灣浮遊細菌和沉積物細菌群落結構和多樣性研究[J]. 生態與農村環境學報,2017,33(11):992-1000.
[17]許豔蕊,方志軍,盧曉平,等. 基于高通量測序技術分析使它隆對玉米土壤細菌多樣性的影響[J]. 微生物學報,2017,57(7):985-993.
[18]余飛宇. 水稻幼苗對镉脅迫的反應及其與全生育期镉積累特性的關系[D]. 揚州:揚州大學,2008.
[19]王錦文,邊才苗,陳珍. 鉛、镉脅迫對水稻種子萌發、幼苗生長及生理指標的影響[J]. 江蘇農業科學,2009(4):77-79.
[20]龍思斯,宋正國,雷鳴,等. 不同外源镉對水稻生長和富集镉的影響研究[J]. 農業環境科學學報,2016,35(3):419-424.
[21]宋建,金鳳媚,薛俊,等. 镉脅迫對植物生長及生理生態效應的研究進展[J]. 天津農業科學,2014,20(12):19-22.
[22]劉小文,齊成媚,歐陽燦斌,等. Pb、Cd及其複合汙染對紫莖澤蘭生長及吸收富集特征的影響[J]. 生態環境學報,2014,23(5):876-883.
[23]朱豔霞,魏幼璋,葉正錢,等. 有機酸在超積累植物重金屬解毒機制中的作用[J]. 西北農林科技大學學報(自然科學版),2006,34(7):121-126.
[24]王秀麗,徐建民,姚槐應,等. 重金屬銅、鋅、镉、鉛複合汙染對土壤環境微生物群落的影響[J]. 環境科學學報,2003,23(1):22-27.
[25]Dunfield K E,Germida J J. Diversity of bacterial communities in the rhizosphere and root interior of field-grown genetically modified Brassica napus[J]. FEMS Microbiology Ecology,2001,38(1):1-9.
[26]Heuer H,Kroppenstedt R M,Lottmann J,et al. Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere relative to communities are negligible natural factors[J]. Applied and Environmental Microbiology,2002,68(3):1325-1335.
[27]劉明,聶菁,劉紫君,等. 轉基因抗病玉米對土壤可培養細菌的影響[J]. 山西農業科學,2016,44(3):353-356.
[28]Griffiths B S,Caul S,Thompson J,et al. Soil microbial and faunal community responses to Bt maize and insecticide in two soils[J]. Journal of Environmental Quality,2006,35(3):734-741.
[29]Lin C H,Pan T M. PCR-denaturing gradient gel electrophoresis analysis to assess the effects of a genetically modified cucumber mosaic virus-resistant tomato plant on soil microbial communities[J]. Applied and Environmental Microbiology,2010,76(10):3370-3373.
[30]Marschner P,Crowley D,Yang C H. Development of specific rhizosphere bacterial communities in relation to plant species,nutrition and soil type[J]. Plant and Soil,2004,261(1/2):199-208.
[31]李孝剛,劉標,徐文華,等. 轉Bt基因抗蟲棉對土壤微生物群落生物多樣性的影響[J]. 生態與農村環境學報,2011,27(1):17-22.

相似文獻/References:

[1]馬淩霄,雷闖,劉紅雙,等.轉OsCYP2基因水稻後代的遺傳檢測及生理特性[J].江蘇農業科學,2014,42(05):98.
 Ma Lingxiao,et al.Genetic testing and physiological characteristics of OsCYP2 transgenic rice offspring[J].,2014,42(14):98.
[2]楊瑞芳,白建江,方軍,等.轉cry1Ac1基因抗蟲水稻和非轉基因親本的主要營養成分比較[J].江蘇農業科學,2016,44(10):328.
 Yang Ruifang,et al.Comparative analysis of nutritional components between insect-resistant rice (Oryza sativa L.) with a synthetic cry1Ac1 gene and its non-transgenic counterpart[J].,2016,44(14):328.
[3]葛慧雯,李佳佳,王高華,等.利用HiTAIL-PCR技術鑒定T-DNA在RNAi-OsRhoGAP2水稻中的插入位點[J].江蘇農業科學,2018,46(09):55.
 Ge Huiwen,et al.Identification of T-DNA insertion site in RNAi-OsRhoGAP2 rice by HiTAIL-PCR[J].,2018,46(14):55.
[4]林萍,高明清,陳永明.基于近紅外光譜分析技術的轉Bt基因水稻種子及其親本快速鑒別方法[J].江蘇農業科學,2019,47(13):72.
 Lin Ping,et al.Rapid identification of Bt transgenic rice seeds and their parents based on near infrared spectroscopy[J].,2019,47(14):72.
[5]杜京堯,尚飛,王高華,等.OsRhoGDI2過表達轉基因水稻的篩選鑒定及外源基因拷貝數的初步分析[J].江蘇農業科學,2019,47(14):50.
 Du Jingyao,et al.Screening and identification of OsRhoGDI2 overexpressed transgenic rice and preliminary analysis of copy number of foreign gene[J].,2019,47(14):50.

備注/Memo

備注/Memo:
收稿日期:2018-03-26
基金項目:轉基因生物新品種培育重大專項(編號:2016ZX08012005)。
作者簡介:李磊(1992—),女,江蘇鹽城人,碩士研究生,主要從事土壤生態與環境微生物多樣性研究。E-mail:151302142@stu.njnu.edu.cn。
通信作者:鍾文輝,博士,教授,主要從事土壤與環境微生物研究。Tel:(025)85891352;E-mail:zhongwenhui@njnu.edu.cn。
更新日期/Last Update: 2019-07-20