|本期目錄/Table of Contents|

[1]杜京堯,尚飛,王高華,等.OsRhoGDI2過表達轉基因水稻的篩選鑒定及外源基因拷貝數的初步分析[J].江蘇農業科學,2019,47(14):50-54.
 Du Jingyao,et al.Screening and identification of OsRhoGDI2 overexpressed transgenic rice and preliminary analysis of copy number of foreign gene[J].,2019,47(14):50-54.
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OsRhoGDI2過表達轉基因水稻的篩選鑒定
及外源基因拷貝數的初步分析
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《江蘇農業科學》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期數:
2019年第14期
頁碼:
50-54
欄目:
生物技術
出版日期:
2019-08-10

文章信息/Info

Title:
Screening and identification of OsRhoGDI2 overexpressed transgenic rice and preliminary analysis of copy number of foreign gene
作者:
杜京堯 尚飛 王高華 梁衛紅
河南師範大學生命科學學院,河南新鄉 453007
Author(s):
Du Jingyaoet al
關鍵詞:
OsRhoGDI2轉基因水稻表達分析拷貝數外源基因
Keywords:
-
分類號:
S511.03
DOI:
-
文獻標志碼:
A
摘要:
水稻Rho GDP解離抑制基因OsRhoGDI2是從幼穗中分離出的功能未知基因。爲鑒定該基因的功能,筆者所在實驗室前期構建了植物過表達載體pCAMBIA1302-OsRhoGDI2-GFP,並對水稻進行了遺傳轉化。對OsRhoGDI2過表達轉基因水稻T2代進行篩選和鑒定,采用PCR技術鑒定轉基因植株,采用半定量RT-PCR和實時熒光定量PCR檢測OsRhoGDI2在轉基因水稻中的表達水平,結果顯示,其中6個株系爲過表達轉基因植株,OsRhoGDI2表達水平上調1.69~13.35倍。爲檢測外源基因在轉基因水稻中的拷貝數,分別以蔗糖磷酸合成酶基因SPS和潮黴素抗性基因HYG爲內參基因和標記基因,采用實時熒光定量PCR(qPCR)技術結合內參基因和標記基因的標准曲線進行分析,結果顯示在所檢測的6個轉基因株系中,外源基因的拷貝數均爲1,提示已經獲得穩定遺傳的OsRhoGDI2過表達轉基因水稻,爲後續OsRhoGDI2基因的功能研究奠定基礎。
Abstract:
-

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備注/Memo

備注/Memo:
收稿日期:2018-03-13
基金項目:國家自然科學基金(編號:31171182、U1704101);河南省高校科技創新團隊支持計劃(編號:15IRTSTHN020)。
作者簡介:杜京堯(1990—),男,河南禹州人,碩士研究生,主要從事植物發育分子生物學研究。E-mail:dujingyao1990@126.com。
通信作者:梁衛紅,博士,教授,主要從事植物發育分子生物學研究。Tel:(0373)3326340;E-mail:liangwh@htu.cn。
更新日期/Last Update: 2019-07-20